JoVE Logo
Faculty Resource Center

Sign In

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation

DOI :

10.3791/52912-v

July 3rd, 2015

July 3rd, 2015

10,885 Views

1Radiological Protection Research and Instrumentation, Canadian Nuclear Laboratories

A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.

Tags

Measuring DNA Damage

-- Views

Related Videos

article

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

article

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

article

Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems

article

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

article

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

article

A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening

article

Measuring Glutathione-induced Feeding Response in Hydra

article

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

article

Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner

article

In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved