July 3rd, 2015
•A protocol to evaluate changes in DNA damage levels and DNA repair capacity that may be induced by chronic in vivo low dose irradiation in mouse spleen lymphocytes, by measuring phosphorylated histone H2AX, a marker of DNA double-strand breaks, using flow cytometry is presented.
Tags
Related Videos
Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems
Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
A Highly Reproducible and Straightforward Method to Perform In Vivo Ocular Enucleation in the Mouse after Eye Opening
Measuring Glutathione-induced Feeding Response in Hydra
Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved