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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

DOI :

10.3791/53158-v

20:00 min

October 31st, 2015

October 31st, 2015

13,361 Views

1Institute of Applied Physics - Biophysics, Vienna University of Technology, 2Institute for Hygiene and Applied Immunology, Immune Recognition Unit, Medical University of Vienna

Preparation of protein-functionalized planar glass-supported lipid bilayers, determination of protein mobility within and measurement of protein densities is shown here. A roadmap to building a noise-reduced Total Internal Reflection microscope is outlined, which allows visualizing single bilayer-resident fluorochromes with high spatiotemporal resolution.

Tags

Single Molecule Fluorescence Microscopy

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