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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

DOI :

10.3791/51794-v

October 1st, 2014

October 1st, 2014

10,061 Views

1Biomedical Engineering, Laboratory for Fluorescence Dynamics, University of California, Irvine

In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.

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3D Orbital Tracking

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