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Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

DOI :

10.3791/58756-v

January 21st, 2019

January 21st, 2019

6,324 Views

1UKE Microscopy Imaging Facility, University Medical Center Hamburg-Eppendorf

An advanced microscope that permit fast and high-resolution imaging of both, the isolated plasma membrane and the surrounding intracellular volume, will be presented. The integration of spinning disk and total internal reflection fluorescence microscopy in one setup allows live imaging experiments at high acquisition rates up to 3.5 s per image stack.

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Spinning Disk TIRF Microscopy

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