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Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP

DOI :

10.3791/53199-v

September 6th, 2015

September 6th, 2015

12,878 Views

1National Institute on Drug Abuse, National Institutes of Health

Endoplasmic reticulum calcium homeostasis is disrupted in diverse pathologies. A secreted ER calcium monitoring protein (SERCaMP) reporter can be used to detect disruptions in the ER calcium store. This protocol describes the use of a Gaussia luciferase SERCaMP to examine ER calcium homeostasis in vitro and in vivo.

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Endoplasmic Reticulum

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