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Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

DOI :

10.3791/53295-v

9:04 min

December 25th, 2015

December 25th, 2015

10,090 Views

1MRC Centre for Regenerative Medicine, School of Biological Sciences, University of Edinburgh

We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

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Localized Transfer

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