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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

DOI :

10.3791/57431-v

9:20 min

April 27th, 2018

April 27th, 2018

13,022 Views

1Centre for Discovery Brain Sciences, University of Edinburgh

We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.

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Isolation

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