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Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

DOI :

10.3791/64572-v

9:16 min

February 3rd, 2023

February 3rd, 2023

1,441 Views

1Schepens Eye Research Institute of Mass. Eye and Ear, 2Department of Ophthalmology, Harvard Medical School

The metabolic status of human retinal pigment epithelial cells (H-RPE) reflects their health and function. Presented here is an optimized protocol for examining the real-time metabolic flux of H-RPE using high-resolution respirometry.

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Real time Analysis

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