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Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

DOI :

10.3791/53424-v

7:45 min

November 28th, 2015

November 28th, 2015

34,174 Views

1Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, Amsterdam

Metabolic reprogramming is a characteristic and prerequisite for M1 and M2 macrophage polarization. This manuscript describes an assay for the measurement of fundamental parameters of glycolysis and mitochondrial function in mouse bone marrow-derived macrophages. This tool can be applied to investigate how particular factors affect the macrophage’s metabolism and phenotype.

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Metabolic Characterization

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