A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

8.5K Views

•

08:22 min

•

December 1st, 2017

DOI :

10.3791/53570-v

December 1st, 2017

•

¹University of Florence, ²INSERM INMED, ³Aix-Marseille University, ⁴Plateforme Biologie Moléculaire et Cellulaire INMED, ⁵Royal Children's Hospital, ⁶Murdoch Children's Research Institute, ⁷University of Melbourne, ⁸Plateforme postgenomique INMED, ⁹University of Pavia, ¹⁰Wellcome Trust Centre for Human Genetics, ¹¹Oxford Radcliffe NHS Trust, ¹²IRCCS Casimiro Mondino Foundation, ¹³Research Institute of Molecular Pathology, ¹⁴IRCCS Stella Maris, ¹⁵Columbia University

Summary

Explore More Videos

Keywords Array CGH

Whole exome Sequencing

In Utero Electroporation

Brain Malformations

Neurobiology

C6orf70 Gene

Periventricular Nodular Heterotopia

Chapters in this video

0:05

Title

1:07

In Utero Electroporation

3:46

Brain Sectioning

5:12

Confocal Imaging and Quantitative Analysis

6:35

Results: C6orf-70-knockdown Alters Radial Migration of Cortical Neurons

7:46

Conclusion

Related Videos

11:51

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

15.0K Views

11:02

Combining Transcranial Magnetic Stimulation and fMRI to Examine the Default Mode Network

12.8K Views

13:12

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

19.8K Views

09:30

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

42.8K Views

06:30

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

17.9K Views

08:44

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

13.4K Views

05:46

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

21.2K Views

11:09

Deep Brain Stimulation with Simultaneous fMRI in Rodents

14.0K Views

07:51

Acrylic Resin Molding Based Head Fixation Technique in Rodents

11.7K Views

10:49

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

9.5K Views