In Utero Electroporation of Multiaddressable Genome-Integrating Color (MAGIC) Markers to Individualize Cortical Mouse Astrocytes
5.4K Views
•
06:47 min
•
May 21st, 2020
DOI :
May 21st, 2020
•Laura Dumas*1, Solène Clavreul*2, Jason Durand1, Edwin Hernandez-Garzon3, Lamiae Abdeladim4, Raphaëlle Barry-Martinet2, Alicia Caballero-Megido1, Emmanuel Beaurepaire4, Gilles Bonvento3, Jean Livet2, Karine Loulier1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
Chapters in this video
0:04
Introduction
0:43
Surgical Preparation
1:39
Pregnant Mouse Preparation
2:16
Intraventricular Injection
3:16
In Utero Electroporation (IUE)
4:24
Multichannel Confocal Imaging
5:23
Results: Representative Cortical Astrocyte Segmentation and Arborization
6:10
Conclusion
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