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Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

DOI :

10.3791/53604-v

February 5th, 2016

February 5th, 2016

8,392 Views

1Medical Faculty Mannheim, Department of Cell and Molecular Biology, Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg, Heidelberg University, 3Excellenzcluster CellNetworks, University of Heidelberg

We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.

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