JoVE Logo
Faculty Resource Center

Sign In

Single-cell Photoconversion in Living Intact Zebrafish

DOI :

10.3791/57024-v

March 19th, 2018

March 19th, 2018

5,814 Views

1Department of Biological Sciences, University of Notre Dame, 2Center for Stem Cells and Regenerative Medicine, University of Notre Dame

Here, we present a protocol to show how cell photoconversion is achieved through UV exposure to specific areas expressing the fluorescent protein, Eos, in living animals.

Tags

Photoconversion Technique

-- Views

Related Videos

article

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

article

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

article

Imaging Subcellular Structures in the Living Zebrafish Embryo

article

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

article

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

article

Induction of Hypoxia in Living Frog and Zebrafish Embryos

article

Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo

article

Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells

article

Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles

article

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved