JoVE Logo
Faculty Resource Center

Sign In

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

DOI :

10.3791/50307-v

10:41 min

July 24th, 2014

July 24th, 2014

13,410 Views

1Max Planck Institute of Molecular Cell Biology and Genetics

Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.

Tags

Keywords Zebrafish

-- Views

Related Videos

article

Single Cell Fate Mapping in Zebrafish

article

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina

article

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin

article

Generation of Mice Derived from Induced Pluripotent Stem Cells

article

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

article

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

article

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

article

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

article

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

article

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved