Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

13.2K Views

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07:40 min

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June 29th, 2020

DOI :

10.3791/60008-v

June 29th, 2020

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Stephanie Mauti¹, Monique Léchenne², Service Naïssengar³, Abdallah Traoré⁴, Vessaly Kallo⁵^,⁶, Casimir Kouakou⁷, Emmanuel Couacy-Hymann⁷, Morgane Gourlaouen⁸, Céline Mbilo⁹^,¹⁰, Pati Patient Pyana¹¹, Enos Madaye³, Ibrahima Dicko⁴, Pascal Cozette¹, Paola De Benedictis⁸, Hervé Bourhy¹, Jakob Zinsstag⁹^,¹⁰, Laurent Dacheux¹

¹Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, ²Environment and Sustainability Institute, University of Exeter, Penryn Campus, ³Institut de Recherche en Elevage pour le Développement, ⁴Laboratoire Central Vétérinaire, ⁵Direction des Services Vétérinaires, ⁶Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, ⁷Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, ⁸FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, ⁹Swiss Tropical and Public Health Institute, ¹⁰University of Basel, ¹¹Institut National de Recherche Biomédicale

Summary

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Postmortem Diagnosis

Rabies

Rapid Immunochromatographic Test

Molecular Applications

Resource limited Settings

Rural Diagnostics

Technique Demonstration

Technician Simon Bonas

Technician Ibrahima Dicko

Foramen Magnum

Brain Tissue Aspiration

Homogeneous Suspension

Test Device Reading

Chapters in this video

0:04

introduction

0:54

Sample Collection and Modified Rabies Rapid Immunochromatographic Diagnostic test (RIDT) Execution

3:46

RNA Extraction and Detection

5:28

Results: Representative RIDT Testing

6:58

Conclusion

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