Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

DOI :

10.3791/60008-v

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7:40 min

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June 29th, 2020

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12,399 Views

Stephanie Mauti¹, Monique Léchenne², Service Naïssengar³, Abdallah Traoré⁴, Vessaly Kallo^5,6, Casimir Kouakou⁷, Emmanuel Couacy-Hymann⁷, Morgane Gourlaouen⁸, Céline Mbilo^9,10, Pati Patient Pyana¹¹, Enos Madaye³, Ibrahima Dicko⁴, Pascal Cozette¹, Paola De Benedictis⁸, Hervé Bourhy¹, Jakob Zinsstag^9,10, Laurent Dacheux¹

¹Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, ²Environment and Sustainability Institute, University of Exeter, Penryn Campus, ³Institut de Recherche en Elevage pour le Développement, ⁴Laboratoire Central Vétérinaire, ⁵Direction des Services Vétérinaires, ⁶Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, ⁷Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, ⁸FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, ⁹Swiss Tropical and Public Health Institute, ¹⁰University of Basel, ¹¹Institut National de Recherche Biomédicale

Chapters

We present a complete protocol for postmortem diagnosis of animal rabies under field conditions using a rapid immunochromatographic diagnostic test (RIDT), from brain biopsy sampling to final interpretation. We also describe further applications using the device for molecular analysis and viral genotyping.

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