Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
9.6K Views
•
09:09 min
•
December 17th, 2015
DOI :
December 17th, 2015
•1Department of Physics and Randall Division of Cell and Molecular Biophysics, King's College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease, King's College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine, University of New South Wales Australia, 4Departments of Chemistry and Physic, McGill University
Explore More Videos
Chapters in this video
0:05
Title
0:50
Microscope Set-up
3:20
Cell Imaging
4:44
Structured Illumination Microscopy (SIM) Image Reconstruction and Spatio-temporal Image Correlation Spectroscopy (STICS) Analysis
7:12
Results: Representative Structured Illumination Data and STICS Output
8:36
Conclusion
Related Videos
16:10
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
23.8K Views
10:31
Real-time Live Imaging of T-cell Signaling Complex Formation
13.7K Views
09:24
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
7.9K Views
10:03
Rapid and Robust Analysis of Cellular and Molecular Polarization Induced by Chemokine Signaling
8.0K Views
09:25
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
8.9K Views
08:44
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
3.3K Views
08:35
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
11.4K Views
11:00
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy
13.7K Views
09:44
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
8.1K Views
06:51
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
7.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved