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Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

DOI :

10.3791/55345-v

January 7th, 2019

January 7th, 2019

10,879 Views

1Institute of Immunology, Section Molecular Immunology, University of Heidelberg

Here, we describe a complete workflow for the qualitative and quantitative analysis of immune synapses between primary human T cells and antigen-presenting cells. The method is based on imaging flow cytometry, which allows the acquisition and evaluation of several thousand cell images within a relatively short period of time.

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Qualitative Analysis

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