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Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy

DOI :

10.3791/57028-v

April 9th, 2018

April 9th, 2018

13,294 Views

1Department of Microbiology and Immunology, University of British Columbia

We present a protocol for using STED microscopy to simultaneously image actin structures, microtubules, and microtubule plus-end binding proteins in B cells that have spread on coverslips coated with antibodies to the B-cell receptor, a model for the initial phase of immune synapse formation.

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Actin

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