JoVE Logo
Faculty Resource Center

Sign In

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors

DOI :

10.3791/53867-v

March 15th, 2016

March 15th, 2016

9,493 Views

1Tumour Angiogenesis Program, Peter MacCallum Cancer Centre

We describe a simple cell-based bioassay for detecting, quantifying and monitoring the activity of members of the vascular endothelial growth factor family of ligands. The assay uses chimeric receptors expressed in a factor-dependent cell line to provide a semi-quantitative or quantitative assessment of receptor binding and cross-linking by the ligand.

Tags

Simple Bioassay

-- Views

Related Videos

article

A Simple Protocol for Extracting Hemocytes from Wild Caterpillars

article

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

article

Isolation of Murine Valve Endothelial Cells

article

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

article

Isolation of Murine Coronary Vascular Smooth Muscle Cells

article

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

article

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

article

Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly

article

Demonstrating a Linear Relationship Between Vascular Endothelial Growth Factor and Luteinizing Hormone in Kidney Cortex Extracts

article

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved