Preparation of WEHI-3D-Conditioned Medium Containing IL-3
2:03
Culture and Evaluation of VEGFR-2-EpoR-Ba/F3 and Control Ba/F3 Cell Lines
4:09
Semi-quantitative Assay
6:17
Quantitative Bioassay
6:59
Results: Inhibition of the Effects of VEGF-A165 by Bevacizumab
8:12
Conclusion
필기록
The overall goal of this simple, cell-based bioassay is to detect, quantify, and monitor the activity of members of the vascular endothelial growth factor family of ligins. This method can answer key questions in vascular biology, virology, cancer
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We describe a simple cell-based bioassay for detecting, quantifying and monitoring the activity of members of the vascular endothelial growth factor family of ligands. The assay uses chimeric receptors expressed in a factor-dependent cell line to provide a semi-quantitative or quantitative assessment of receptor binding and cross-linking by the ligand.