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Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

DOI :

10.3791/53936-v

7:17 min

April 14th, 2016

April 14th, 2016

78,899 Views

1Department of Urology, Johns Hopkins University

Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.

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Murine Hind Limb

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