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Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry

DOI :

10.3791/54157-v

July 20th, 2016

July 20th, 2016

35,231 Views

1School of Chemistry, The Australian Centre for Nanomedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales

This protocol details the important steps required for the bioconjugation of a cysteine containing protein to a maleimide, including reagent purification, reaction conditions, bioconjugate purification and bioconjugate characterization.

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