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Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

DOI :

10.3791/62419-v

May 10th, 2021

May 10th, 2021

3,363 Views

1Cardiovascular Research Center, The Icahn School of Medicine at Mount Sinai, 2Diabetes, Obesity and Metabolism Institute, Department of Medicine, The Icahn School of Medicine at Mount Sinai, 3Graduate School of Biomedical Sciences, The Icahn School of Medicine at Mount Sinai

This article describes the isolation of mouse aortic valve cells by a two-step collagenase procedure. Isolated mouse valve cells are important for performing different assays, such as this in vitro calcification assay, and for investigating the molecular pathways leading to aortic valve mineralization.

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