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Neuroscience

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Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure

8.7K Views

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00:09 min

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August 27th, 2019

DOI :

10.3791/59234-v

August 27th, 2019

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Honghui Mao*¹^,², Xiuchao Wang*¹^,³, Wen Chen⁴, FengYu Liu⁵, You Wan⁵, Sanjue Hu¹, Junling Xing¹^,⁶

¹Department of Neurobiology, School of Basic Medicine, Fourth Military Medical University, ²Department of Toxicology, School of Public Health, ShanXi Medical University, ³Department of Psychology, Fourth Military Medical University, ⁴Department of Neurobiology, School of Basic Medical Sciences, Advanced Innovation Center for Human Brain Protection, Capital Medical University, ⁵Neuroscience Research Institute, Key Lab for Neuroscience, Ministry of Education/National Health Commission, Peking University, ⁶Department of Radiation Biology, Faculty of Preventive Medicine, Fourth Military Medical University

Summary

Single-fiber recording is an effective electrophysiological technique that is applicable to the central and peripheral nervous systems. Along with the preparation of intact DRG with the attached sciatic nerve, the mechanism of conduction failure is examined. Both protocols improve the understanding of the peripheral nervous system's relationship with pain.

Explore More Videos

In Vivo Single fiber Recording

Dorsal Root Ganglion

Conduction Failure

Peripheral Sensation

Integrated DRG Tests

Surgical Preparation

Normal Ringer s Solution

Anesthetized Rat

Blunt Dissection

Electrophysiology

Recording Electrode

Stereo microscope

Receptive Field

Nonconceptive C Fiber

Chapters in this video

0:04

Title

1:13

In Vivo Single-fiber Recording

4:16

Preparation of Intact DRG Attached with Sciatic Nerve

7:48

Results: Attenuation of Conduction Failure in CFA-treated Rats and Exploration of AHP Property

8:55

Conclusion

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