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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

DOI :

10.3791/4141-v

August 17th, 2012

August 17th, 2012

26,201 Views

1Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine

An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

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Genetic Study

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