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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

DOI :

10.3791/54114-v

May 8th, 2016

May 8th, 2016

25,988 Views

1Division of Infectious Diseases, Department of Medicine, Boston Children’s Hospital

There is growing interest in the quantitative characterization of intestinal lymphocytes owing to increasing recognition that these cells play a critical role in a variety of intestinal and systemic diseases. In this protocol, we describe how to isolate single cell populations from different small-intestinal compartments for subsequent flow cytometric characterization.

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