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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

DOI :

10.3791/64338-v

6:14 min

November 30th, 2022

November 30th, 2022

1,300 Views

1Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine, University of Pittsburgh

The present protocol describes a detailed surgical protocol for isolating flowing intestinal lymph in response to intraduodenal nutrient infusions in mice. This allows for the physiological determination of total intestinal lipid absorption and chylomicron synthesis and secretion in response to various experimental nutrients.

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Flowing Mesenteric Lymph

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