JoVE Logo
Faculty Resource Center

Sign In

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

DOI :

10.3791/54161-v

July 13th, 2016

July 13th, 2016

65,032 Views

1Department of Medicine, Stony Brook University School of Medicine, 2Department of Physiology & Biophysics, Stony Brook University School of Medicine

Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.

Tags

Swiss rolling Technique

-- Views

Related Videos

article

Pressure-polishing Pipettes for Improved Patch-clamp Recording

article

Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue

article

Evisceration of Mouse Vitreous and Retina for Proteomic Analyses

article

An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells

article

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

article

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

article

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

article

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

article

Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)

article

Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved