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Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

DOI :

10.3791/54161-v

July 13th, 2016

July 13th, 2016

62,837 Views

1Department of Medicine, Stony Brook University School of Medicine, 2Department of Physiology & Biophysics, Stony Brook University School of Medicine

Accurate identification and location of epithelial cells along the intestinal mucosal lining are essential to define different cell lineages. Proper imaging of intestinal tissues is crucial for identification of protein expression patterns with maximum resolution. This study aims to delineate the optimal methods and conditions for processing mouse intestinal tissues.

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