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A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

DOI :

10.3791/52531-v

April 20th, 2015

April 20th, 2015

29,432 Views

1Tytgat Institute for Liver and Intestinal Research, Department of Gasteroenterology and Hepatology, Academical Medical Center

In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.

Tags

Keywords Intestinal Organoids

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