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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

11.1K Views

14:53 min

October 24th, 2016

DOI :

10.3791/54227-v

October 24th, 2016


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Adaptive Evolution

Chapters in this video

0:05

Title

1:40

Enrichment of Robust Derivatives during Serial Transfer on AFEX CSH

3:42

Isolation of Single Cell Tolerant Derivatives after Enrichment on AFEX CSH

4:58

Performance Evaluation of AFEX CSH Tolerant Derivatives Compared to Parent

7:15

Applying Continuous Culture to Select for Ethanol-challenged Xylose Utilization

9:43

Isolation of Single-cell Colonies That Utilize Xylose in PSGHL When Ethanol Is Present

11:20

Results: Derivatives of S. stipitis Parent Strain Have Improved Efficiency in Converting Hydrolyzates to Ethanol

12:54

Conclusion

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