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Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

DOI :

10.3791/54271-v

12:43 min

July 27th, 2016

July 27th, 2016

10,907 Views

1Wales Heart Research Institute, Cardiff University School of Medicine, College of Biomedical and Life Sciences

Oligomerization of the ryanodine receptor, a homo-tetrameric ion channel mediating Ca2+ release from intracellular stores, is critical for skeletal and cardiac muscle contraction. Here, we present complementary in vivo and in vitro methods to detect protein self-association and determine homo-oligomer stoichiometry.

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Genetic Approaches

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