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Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

DOI :

10.3791/50180-v

16:07 min

March 30th, 2013

March 30th, 2013

19,980 Views

1Center for Human Genetic Research and Department of Medicine, Massachusetts General Hospital and Harvard Medical School, 2Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology

We present robust biochemical and microscopic methods for studying Caenorhabditis elegans lipid stores. A rapid, simple, fixing-staining procedure for fluorescent lipid droplet imaging leverages the spectral properties of the lipophilic dye Nile red. We then present biochemical measurement of triglycerides and phospholipids using solid phase extraction and gas chromatography-mass spectrometry.

Tags

Biochemical Assessment

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