JoVE Logo

Sign In

In vitro Methylation Assay to Study Protein Arginine Methylation

15.2K Views

10:01 min

October 5th, 2014

DOI :

10.3791/51997-v

October 5th, 2014


Explore More Videos

Protein Arginine Methylation

Chapters in this video

0:05

Title

1:54

Purification of Protein Arginine Methyl Transferases (PRMTs)

5:31

Methylation Assay

7:16

Confirmation of PRMT Expression and Equal Loading of Substrate

8:38

Results: PRMT1 Methylates Ras-GTPase Activating Protein Binding Protein 1 (G3BP1)

9:40

Conclusion

Related Videos

article

12:30

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards

11.5K Views

article

08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

7.2K Views

article

08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

6.5K Views

article

07:02

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

1.4K Views

article

Proximity Ligation Assay Allows the Detection, Localization, and Quantification of Protein Arginine Methylation in Fixed Tissue

1.0K Views

article

09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

2.2K Views

article

09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

17.4K Views

article

08:38

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

22.0K Views

article

12:34

DNA Methylation: Bisulphite Modification and Analysis

104.6K Views

article

08:11

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

4.1K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved