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Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

DOI :

10.3791/54303-v

October 7th, 2016

October 7th, 2016

7,954 Views

1Department of Molecular and Cellular Physiology, Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics, Albany Medical College

We describe a protocol to volumetrically image fluorescent protein labeled cells deep inside intact embryonic and postnatal hearts. Utilizing tissue-clearing methods in combination with whole mount staining, single fluorescent protein-labeled cells inside an embryonic or postnatal heart can be imaged clearly and accurately.

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