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Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos

DOI :

10.3791/62741-v

7:29 min

August 13th, 2021

August 13th, 2021

3,257 Views

1Morgridge Institute for Research, Madison, WI

We describe optimized tools to study the zebrafish heart in vivo with light sheet fluorescence microscopy. Specifically, we suggest bright cardiac transgenic lines and present new gentle embedding and immobilization techniques that avoid developmental and heart defects. A possible data acquisition and analysis pipeline adapted to cardiac imaging is also provided.

Tags

Light Sheet Microscopy

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