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Microinjection-based System for In Vivo Implantation of Embryonic Cardiomyocytes in the Avian Embryo

DOI :

10.3791/59267-v

8:52 min

February 17th, 2019

February 17th, 2019

5,785 Views

1Department of Cell Biology and Physiology, McAllister Heart Institute, University of North Carolina at Chapel Hill

In this method, embryonic cardiac tissues are surgically microdissected, dissociated, fluorescently labeled, and implanted into host embryonic tissues. This provides a platform for studying the individual or tissue level developmental organization under ectopic hemodynamic conditions, and/or altered paracrine/juxtacrine environments.

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Microinjection based System

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