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A Method of Targeted Cell Isolation via Glass Surface Functionalization

DOI :

10.3791/54315-v

September 20th, 2016

September 20th, 2016

9,145 Views

1Department of Bioengineering, University of Illinois at Urbana-Champaign, 2Department of Liberal Arts & Sciences, University of Illinois at Urbana-Champaign, 3Department of Biomedical Engineering, Illinois Institute of Technology

This protocol describes customizable surface functionalization of the desthiobiotin, streptavidin, and APTES system in order to isolate specific cell types of interest. In addition, this manuscript covers the applications, optimization, and verification of this process.

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