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A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy

DOI :

10.3791/51873-v

September 16th, 2014

September 16th, 2014

13,354 Views

1Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign

Traditional techniques for fabricating polyacrylamide (PA) gels containing fluorescent probes involve sandwiching a gel between an adherent surface and a glass slide. Here, we show that coating this slide with poly-D-lysine (PDL) and fluorescent probes localizes the probes to within 1.6 µm from the gel surface.

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