SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

10.8K Views

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10:58 min

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August 24th, 2016

DOI :

10.3791/54349-v

August 24th, 2016

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Joerg Nikolaus¹^,², Erdem Karatekin¹^,²^,³^,⁴

¹Department of Cellular and Molecular Physiology, Yale University School of Medicine, ²Nanobiology Institute, Yale University, ³Department of Molecular Biophysics and Biochemistry, Yale University, ⁴Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales, Centre National de la Recherche Scientifique (CNRS)

Summary

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Keywords SNARE mediated

Proteoliposomes

Supported Bilayers

Microfluidic Flow Cell

Polarized TIRF Microscopy

Membrane Fusion

Neurotransmitter Release

Chapters in this video

0:05

Title

1:01

Preparation of a PDMS Block to Form the Microfluidic Channel

3:17

Formation of the Tethered Supported Bilayer

6:18

Checking Bilayer Fluidity

7:06

Introducing v-SUVs into the Microfluidic Channels

7:59

Observing the Fusion of Single v-SUVs and the SBL

8:42

Results: Docking and Fusion Events Described by Lipid and Content Release

10:26

Conclusion

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