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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

DOI :

10.3791/54389-v

July 27th, 2016

July 27th, 2016

9,263 Views

1Department of Biochemistry, Brandeis University, 2Winship Cancer Institute, Emory University School of Medicine

The Tandem Affinity Purification (TAP) method has been used extensively to isolate native complexes from cellular extract, primarily eukaryotic, for proteomics. Here, we present a TAP method protocol optimized for purification of native complexes for structural studies.

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