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Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

9.4K Views

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10:36 min

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July 27th, 2016

DOI :

10.3791/54389-v

July 27th, 2016

•
9,414 Views
1Department of Biochemistry, Brandeis University, 2Winship Cancer Institute, Emory University School of Medicine

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Native Macromolecular Complex
Purification
Tandem Affinity Tag
Structural Study
S Cerevisiae
Cell Lysis
Liquid Nitrogen
Coffee Grinder
Centrifugation
Buffer Conditions
Protein Complex
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