February 24th, 2011
•In this video, we describe the characterization of multiprotein complexes (MPCs) by blue native polyacrylamide gel electrophoresis (BN-PAGE). In a first dimension, dialyzed cellular lysates are separated by BN-PAGE to identify individual MPCs. In a second dimension SDS-PAGE, MPCs of interest are further subdivided to analyze their constituents by immunoblotting.
Tags
Related Videos
Denaturing Gradient Gel Electrophoresis (DGGE)
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
Identification of protein complexes with quantitative proteomics in S. cerevisiae
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Pouring and Running a Protein Gel by reusing Commercial Cassettes
Agarose Gel Electrophoresis for the Separation of DNA Fragments
Rapid Generation of Amyloid from Native Proteins In vitro
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved