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GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins

DOI :

10.3791/50320-v

October 29th, 2013

October 29th, 2013

38,819 Views

1Genome Stability Laboratory, Oncology Axis, Hôtel-Dieu de Québec

In the present protocol, we demonstrate a highly efficient and cost-effective small-scale protein purification method, which allows purification of recombinant proteins by uniquely combining a cleavable GST-tag and a small His-tag.

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