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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

DOI :

10.3791/54391-v

January 16th, 2017

January 16th, 2017

8,684 Views

1Department of Veterinary & Biomedical Sciences, University of Minnesota, 2Department of Veterinary Biosciences, Ohio State University, 3School of Biotechnology, Gautam Buddha University

This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.

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