Synthesis and Operating Principles of the 'Chemical Transducer'
1:51
Setting Up the Plate Reader for Kinetic Measurement
3:26
Measuring GST Activity in the Presence of the 'Chemical Transducer' and PDGF
4:59
Evaluating GST Activation/Inhibition Cycles Mediated by the 'Chemical Transducer'
6:37
Evaluating the Real-time Effect of PDGF Addition
7:42
Measuring JS-K Prodrug Activation by GST in the Presence of the 'Chemical Transducer' and PDGF
10:16
Results: 'Chemical Transducer' Induces Artificial Communication Between PDFG and GST
11:53
Conclusion
Transcript
The overall goal of this experiment is to spectroscopically follow the unnatural communication between two proteins induced by a synthetic chemical transducer. The will demonstrate the possibility of developing synthetic analogs of signalling prot
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We present guidelines for developing synthetic 'chemical transducers' that can induce communication between naturally unrelated proteins. In addition, detailed protocols are presented for synthesizing and testing a specific 'transducer' that enables a growth factor to activate a detoxifying enzyme and consequently, to regulate the cleavage of an anticancer prodrug.