October 4th, 2019
•Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.
Related Videos
In Vitro Transcription Assays and Their Application in Drug Discovery
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
Production and Purification of Baculovirus for Gene Therapy Application
Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells
Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved