JoVE Logo
Faculty Resource Center

Sign In

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

DOI :

10.3791/54463-v

9:09 min

September 20th, 2016

September 20th, 2016

10,999 Views

1The Bridge Institute, University of Southern California, 2Department of Chemistry, University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute, Arizona State University

We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

Tags

Keywords Protein Microcrystals

-- Views

Related Videos

article

Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics

article

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

article

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

article

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

article

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

article

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

article

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

article

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

article

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

article

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved