JoVE Logo
Faculty Resource Center

Sign In

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

DOI :

10.3791/54529-v

September 20th, 2016

September 20th, 2016

12,283 Views

1Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 2Department of Oncology, Johns Hopkins University School of Medicine

We describe here a method for identification of small molecule-binding proteins using photoaffinity labeling. The advantage of this technique is that binding and covalent labeling of the target proteins occurs within the live cellular environment, removing the risk of disrupting native protein structure and binding conditions upon cell lysis.

Tags

Small Molecule binding Proteins

-- Views

Related Videos

article

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

article

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

article

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis

article

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

article

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

article

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

article

Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes

article

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

article

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

article

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved