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Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

DOI :

10.3791/53157-v

19:05 min

October 30th, 2015

October 30th, 2015

11,877 Views

1Institute for Applied Physics - Biophysics, Vienna University of Technology, 2Institute for Hygiene and Applied Immunology, Medical University of Vienna

This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.

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TCR pMHC Binding

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