The overall goal of this experiment is to visualize the micro-structure of, and cell adhesion on, a non-transparent knitted titanium nucleus implant, using fluorescent imaging techniques. This method advances the analysis of tissue engineering wit
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Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.